Looking for a reliable and user-friendly software to manage your Windows 7 system? Look no further than FONZIE, developed by software guru Jonathan Grandaubert. With its sleek design and intuitive interface, FONZIE provides an easy way to optimize, customize, and maintain your Windows 7 system. Whether you're a tech expert or just getting started, FONZIE is the perfect solution for managing all your computer needs. So why wait? Download FONZIE today and experience the ultimate in Windows 7 software management!

FONZIE was designed to successively 1) perform a search for markers via the external software Tandem Repeat Finder, 2) exclude user-defined specific genomic regions, 3) screen for the size and the percent matches of each relevant marker found by Tandem Repeat Finder, 4) evaluate marker specificity (i.e., occurrence of the marker as a single copy in the genome) using BLAST2.0, 5) design minisatellite primer pairs via the external software Primer3, and 6) check the specificity of each final PCR product by BLAST.

A final file returns to users all the results required to amplify markers. A biological validation of the approach was performed using the whole genome sequence of the phytopathogenic fungus Leptosphaeria maculans, showing that more than 90% of the minisatellite primer pairs generated by the pipeline amplified a PCR product, 44.8% of which showed agarose-gel resolvable polymorphism between isolates. Segregation analyses confirmed that the polymorphic minisatellites corresponded to single-locus markers.

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